Critical Analysis of Mass Spectrometry The WritePass Journal

Critical Analysis of Mass Spectrometry Introduction Critical Analysis of Mass Spectrometry Introduction1. Background:2. Methods of Ionization:3. Mass Analysers:4. The Mass Spectra:5. Mass Spectrometry in Synergy with other Techniques:6. Advances in Mass Spectrometry Instruments and their Limitations:7. The Future of Mass Spectrometry:References:Related Introduction 1. Background: The analytical technique that I have chosen to give an in-depth analysis of is Mass Spectrometry (MS) This analytical technique is basically the study of ionised molecules in the gaseous phase; its main use is in the determination of the molecular weight of the molecule in the sample under investigation by accelerating ions in a vacuum environment. While this analytical technique has been around for over one hundred years there are significant advances being made to this technique in order to cater for more adverse samples which will be discussed in more detail later on. The main difference between mass spectrometry and other spectroscopy methods such as NMR is that it not dependant on transitions between energy states which may be responsible for its popularity. The diagram shown below (Figure 1.1.) [1] shows a simple diagram of a common mass spectrometer using electron ionization:    Figure 1.1 represents a schematic diagram of an electron ionization-mass spectrometer showing the various processes involved. Courtesy of www.molecularstation.com. In its simplest form the process of determining the molecular weight of the sample typically occur over four main stages which are: Sample volatilisation, Ionisation, Separation and detection. Sample volatilisation: The sample to be analysed if gaseous or volatile can be readily inserted into the mass spectrometer with the more solid samples requiring heating before insertation in order to construct a more volatile or gaseous sample. As can be seen form the above figure the sample is then moved further down the spectrometer towards the area where ionization of the molecules occurs.   Ionization: The sample is then hit with a barrage of high energy electrons from an electron gun with a charge of around 70 electron volts (eV). When the molecules collide with the high energy electron beam energy is transferred from the beam to the molecules which cause an acceleration of the molecules. These molecules may then dump an electron forming cation known as the molecular ion (M+) [2]. This interchange is represented in the equation below (Figure 1.2.): M  Ã‚  Ã‚  Ã‚  Ã‚   +  Ã‚  Ã‚  Ã‚   e  Ã‚     Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   M+  Ã‚  Ã‚   +  Ã‚  Ã‚   2e   Moleccular Ion This electron barrage usually results in most of the molecular ions fragmenting causing some of the fragments to not gain any charge and remain neutral and have no further part to play. The main purpose of ionisation is to donate a charge to the sample in order for the molecules to break up and become charged. The ionization method discussed here is electron ionisation however there are many other more methods of ionization which will be discussed in detail later on in my analysis. Separation: This beam of newly charged molecular ions then proceed through a mass analyzer which in this case is a very strong controllable magnetic field which separates the charged molecules according to their mass to charge ratio (m/z) causing some of the molecules which are â€Å"too heavy† or â€Å"too light† to be thrown towards the top or bottom of the spectrometer and hence avoid detection. By varying the magnetic field, ions with different m/z values can be detected. Just like there are many different ionization methods for different applications there are also several types of mass analyzers which will also be discussed later. A fundamental consideration in mass spectrometry at this point is mass resolution, defined as R = M/∆M. where R is the resolution, M is the mass of particle and ∆M is the mass difference compared to adjacent peak with overlap at 10% of peak height. Nowadays a magnetic sector analyzer can have R values of 2000-7000 depending on the instrument [3]. Detection: the final stage in the process is comprised of a detector which then amplifies and records the mass of the ions according to their m/z values. The detector may be set up for detection of molecular ions possessing different mass to charge ratios. The Molecular ions each have a mass that is almost identical to the mass of the molecule (M) and due to the fact that the charges on most of the molecules are usually 1, the value of m/z obtained for each of the ions is simply its mass. The data collected by the detector is fed to a recorder and is presented in the form of a plot of the numbers of ions versus their m/z values [3]. An example of this type of plot is shown below in figure 1.3. [4]: Figure 1.3: A typical graph produced for a sample using mass spectrometry. Picture courtesy of www.research.uky.edu. 2. Methods of Ionization: Electron Ionization (EI): as described above is the simplest method for converting the sample to ions and this method is found on the most common mass spectrometers. Many other simple and complex ionization methods exist for analyzing various samples. Some of these methods include: Chemical Ionization (CI): This is a softer ionization method than EI, causing less fragmentation of the sample under investigation and hence it is mainly used for more sensitive compounds such as 2, 2-dimethylpropane for example which is prone to fragment with little stress. This decrease in fragmentation is due to the ions arising from a chemical reaction rather than bombardment and hence possesses less energy than those produced from EI. In Chemical ionization the molecules to be studied are mixed with an ionized carrier gas which is present in excess. Common carrier gases for CI include ammonia, methane, isobutene and methanol. The selection of the carrier gas depends on the degree of ion fragmentation required. Different carrier gases produce different mass spectra plots. The main advantage of CI is its softer approach lending to clearer results over EI for some samples. Other advantages include the relatively cheap and strong hardware as with EI. The main drawback of using chemi cal ionization in mass spectrometry is the fact that like electron ionization the sample must be readily vaporised in order for the molecules to gain that vital charge. This immediately dismisses the use of high molecular weight compounds and biomolecules [3]. It’s obvious therefore that CI and EI are very similar methods of ionization and due to this many of the modern day mass spectrometers can switch between these two methods effortlessly. Electrospray Ionization (ESI): is a type of atmospheric pressure ionization. This technique is very useful for studying the high biomolecular weight molecules and other samples which may not be very volatile as discussed above. The sample to be investigated is sprayed through a fine capillary which has a charge on its surface, the sample then enters the ionization chamber resulting in the production of multiple charged ions along with single charged ions. This formation of multiple charged ions is very useful in the mass spectrometry analysis of proteins [3]. It is important to note that negative ions may also be formed in ESI and the operation may need to be reversed. ESI has become much more common over the last few years as it relies on a sample in solution which permits its use in LC-MS [5]. Thermospray Ionization (TSI) is closely related to ESI differing only in the fact that it relies on a heated capillary rather than a charged capillary; however ESI remains the more popular of the two methods. Atmospheric-pressure chemical Ionization (APCI): It is obvious form the title that APCI is also a form of atmospheric pressure ionization resulting in a similar interface being used for both methods. This method was born in the 1970’s when it was first combined with liquid chromatography (LC) by Horning et al [6] who conveyed a new atmospheric ion source which used 63Ni beta emission in order to produce the required ions. Even tough APCI and ESI are harmonizing methods the main advantage APCI has over ESI is that it is more effective at determining the mass spectra for less polar compounds due to the reality that the gas phase ionization is more effective in APCI. Many MS instruments are now readily available with high mass resolution and accurate mass measurement, properties which are not as readily available with GC-MS instruments. Fast Atom Bombardment (FAB): this type of ionization method is primarily used for large polar molecules. The sample to be studied is usually dissolved in a liquid matrix which is non-volatile and polar such as glycerol. This sample is then bombarded with a fast atom beam such as Xe atoms which picks up electrons thus causing ionization from this reaction. This is a simple and fast method to use and is very good for high-resolution measurements. On the downside however it may be hard to compare low molecular weight compounds from the chemical back ground which is always high [5]. Desorption Chemical Ionization (DCI), Negative-ion chemical ionization (NCI), Field Ionization (FI) and Ion Evaporation are other less common ionization methods used in mass spectrometry. 3. Mass Analysers: As described earlier the mass analyzers are used to separate the various ions according to their mass to charge ratio (m/z) and hence focus the ions with the desirable m/z value towards the detector. Some of the mass analyzers available include; Double-Focusing Mass Analyzers, Quadrupole Mass Analyzers, Time-of-Flight Mass Analyzers and Ion Trap Mass Analyzers. Double-Focusing Mass Analyzers are used when a high resolution is of paramount importance. This high resolution is achieved by modifying the basic magnetic design. The beam of ions passes through an electrostatic analyser before or after the magnetic field causing the particles to travel at the same velocity resulting in the resolution of the mass analyzer increasing dramatically. Resolution may be varied by using narrower slits before the detector. It is important to note that this type of analyzer reduces sensitivity but increases accuracy resulting in a fine line between success and failure with regards to detection, for this reason this type of mass analyzer is only used for very selective purposes. Quadrupole Mass Analyzers do not make use of magnetic forces for mass detection; instead they are composed of four solid rods arranged parallel to the direction of the ion beam. Using a combination of direct-current and radiofrequency the quadrupole separates the various ions according to their mass extremely quickly. Quadrupole mass analyzers are most on most GC-MS instruments. Time-of-Flight Mass Analyzers (TOF) operate by measuring the time taken for an ion which has been produced to travel for the ion source to the detector [7]. This is based on the simple assumption that the lighter ions will have a greater velocity and thus will strike the detector first. This type of analyzer has become more and more common in recent years due to the fact that the electronics used in this analyzer have become much more affordable since it was first introduced in the 1940’s. In recent years the resolution and sensitivity of TOF have been increased by the insertation of a reflective plate within the flight tube itself [8]. The main area that this type of analyzer is used is in Matrix Assisted Laser Desorption Mass Spectrometry (MALDI-MS) discussed later. The Ion Trap Mass Analyzer is composed of two hyperbolic end cap electrodes and a doughnut shaped ring electrode [7]. It is very similar to the quadrupole analyzer in resolution terms and basics however the ion trap is more sensitive. 4. The Mass Spectra: The main interest that anybody has from the mass spectra is the molecular weight of the sample that was processed. The value of m/z at which the molecular ion (M+) appears on the mass spectrum tell us the molecular weight of the original molecule. The most saturated ion formed from the ionization provides us with the tallest peak in the spectra know as the base peak (Figure 1.2). From this information the determination of very exact molecular weights of substances may be deduced which is probably the most important application of mass spectrometers. This determination also allows use to distinguish between different substances with a very similar molecular mass which we are unable to do ourselves. For example; the molecule C14H14 has a molecular mass of 182.1096 and the molecule C12H10N2 has a molecular mass of 182.0844. These two molecules may only be differentiated by MS as there is only 0.0252 in the difference even tough they are two completely different molecules. The type of MS instrument used in this case is a Double Focusing Mass Spectrometer as discussed briefly above which is capable of providing measurements accurate to 0.0001 atomic mass units. The chance of two compounds having the exact same mass spectra is very unlikely and therefore it is possible to identify an unknown compound by comparing its mass spectra obtained with that of a known library of mass spectra for various compounds. 5. Mass Spectrometry in Synergy with other Techniques: Through the years mass spectrometers have evolved to be used not just on their own but used in tandem with a range of other analytic techniques such as Liquid Chromatography – Mass Spectrometry (LC-MS) in purity assessment and investigating rat urine, Gas Chromatography-Mass Spectrometry (GC-MS) for the detection and measurement of illicit drugs in biological fluids. It is LC-MS that has become the gold standard for detection and analyzation of substances. Gas chromatography works particularly well with mass spectrometry too, due to the face that the sample is already in its gaseous form at the interface. This system has been used by De Martinis and Barnes [9] in the detection of drugs in sweat using a quadrupole mass spectrometer which has been discussed earlier. The ability to identify metabolites in the biological fluids mentioned above can be very difficult and this is due to the fact that these metabolites are present in extremely low concentrations such as parts per mill ion (ppm) or even less in some situations. For many years Nuclear Magnetic Resonance (NMR) was used to identify these metabolites but in recent times it would appear that mass spectrometry has become the more popular method for detection of the metabolite. This may be due to the fact that MS is more sensitive than NMR resulting in less sample amount being required. 6. Advances in Mass Spectrometry Instruments and their Limitations: As mentioned briefly above it is very difficult to study large biomolecules such as proteins due to the fact that they are large polar molecules which are not volatile and as a result are difficult to convert to a gaseous state in order to undergo ionization. In recent years a solution to this problem has been accomplished with the introduction of Matrix- Assisted Laser Desorption Ionization (MALDI). MALDI is a laser based soft ionization method which relies on the sample being dissolved in a solution containing an excess of matrix such as sinapinic acid which has a chromophore that absorbs at the laser wavelength, the sample is placed in the path of high intensity protons causing a collision of the atoms with the sample resulting in ionization of the sample molecules causing them to be ejected from the matrix. One of the main advantages of MALDI-MS is that only a very tiny amount of sample is required (1 X 10-5 moles) [3]. This technique has proven to be one of the most successful ionization methods for mass spectrometry analysis of large molecules due to its soft ionization ability. This technique has been used in the drug-biomolecule complexes in order to investigate the interaction properties and sites of biomolecules with various drugs on the market today [10]. This method was also used by Zschorning et al. to investigate the extracts of human lipoproteins after treatment wi th cholesterol esterase’s [11]. This method although very popular suffers some drawbacks. There is a strong dependence on the sample preparation method and any mistake made during sample preparation or any contamination introduced into the matrix during the sample preparation renders the rest of the investigation pointless. Another draw back of this method is the short sample life although some research has been undertaken [12] with the use of liquid matrices in the belief that this may increase the sample life by making use of the self-healing properties of the sample through molecular diffusion. One obvious drawback that may occur is the fact that the sample may not be soluble and hence may not dissolve in the matrix. This problem may be overcome with the use of compression of a finely ground sample and analyte [13]. Another disadvantage which may become of detrimental in the future is the fact that MALDI is not easily compatible with LC-MS, this problem may have to be rectified id the popularity of MALDI is to c ontinue. Electrospray Ionization (ESI) has been described in detail under the methods of ionization section above and it can be seen that this young technique is proving to be very useful with LC-MS to investigate the a variety of molecules including proteins, DNA and synthetic polymers. The main problem with ESI-MS is that the mass spectra produced may contain many peaks of multiply charged ions which may cause confusion in the interpretation of spectra of some samples. The ESI instrument itself can also present with decreased sensitivity due to the presence of impurities such as salts and buffers, this is not the case with MALDI. Although both MALDI and ESI are both very effective methods of developing mass spectra for large molecules such as proteins, MALDI still remains the method of choice for most analyses. However, as discussed above the fact that MALDI is not very compatible with LC-MS may pave the way for a surge in popularity of the LC-MS friendly ESI. 7. The Future of Mass Spectrometry: Mass spectrometry has come along way since 1897 when Joseph J. Thompson used an early mass spectrometer to discover the electron and there is no reason why the mass spectrometer will continue to advance and evolve into the foreseeable future. The mass spectrometer is an extremely versatile analytical tool which can work in tandem and alongside other analytical methods such as chromatography seamlessly. The main areas in which mass spectrometers have been used for quantification of compounds are LC-MS and GC-MS using the various ionization methods respectively. LC-MS is the gold standard in quantitative bioanalyses and is used by the majority pharmaceutical companies. The other minority tend to use other techniques such as High Pressure Liquid Chromatography (HPLC) and UV as they deem LC-MS to be too expensive. An area of mass spectrometry to watch out for in the future is the use of ion-trap technology to perform LC-MS-MS to LC-MS [7]. This method already exists but reliable routine bioanalytical assays have not been produced as of yet. References: [1]  Ã‚   molecularstation.com/molecular-biology-images/506-molecular-biology-pictures/21-mass-spectrometer.html [2]  Ã‚   Daniel C. Harris: Quantitative Chemical Analysis, sixth edition (2003) published by W. H. Freeman and Company, New York. [3]  Ã‚   Donald L. Pavia, Gary M. Lampman, George S. Kriz and James R. Vyvyan: Introduction to Spectroscopy, fourth edition, published by Brooks/Cole, Cengage Learing. [4]  Ã‚   www.research.uky.edu/ukmsf/whatis.html [5]  Ã‚   Ionization Methods in Organic Mass Spectrometry [6]  Ã‚   Horning, E.C., Caroll, D.J., Dzidic, I., Haegele, K.D., Horning, M.G., and  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   Stillwell,R.N. (1974). Atmospheric pressure ionization (API) mass spectrometry. Solvent-mediated ionization of samples introduced in solution and in a liquid chromatograph effluent stream,   J. Chromatography. Sci, 12, (11), 725-729 [7]  Ã‚   RF Venn (Ed) (2000) Principles and practice of Bioanalysis Taylor and Francis. [8]  Ã‚   Ashcroft, A.E. (1997) Ionisation Methods in Organic Mass Spectrometry, Cambridge,   UK: The Royal Society of Chemistry. [9]  Ã‚   asms.org/whatisms/p1.html: The American Society of Mass Spectrometry [10]  Ã‚   Skelton, R., Dubols, F., Zenobl, R. Analytical Chemistry (2000), 72, 1707-1710 [11]  Ã‚   Zschornig, Markus Pietsch, Roesmarie SuB., Jurgen Schiller and Michael Gutschow. Cholesterol esterase action on human high density lipoproteins and inhibition studies: detection by MALDI-TOF MS.    [12]  Ã‚   Zenobi, R, Knochenmuss, R. Mass Spectrom, Rev. 1999, 17, 337-366.

Free sample - Poes Horror. translation missing

Poes Horror. Poe's HorrorINTRODUCTION Edgar Allan Poe (1809 - 1849) was a famous poet in America who wrote many stories and poems (Hossick 28). He was also an editor as well as a writer and he worked with several journals and publishing homes. He is most famous for his stories of mystery. It is believed that Edgar Allan made a generous contribution to the genre of scientific fiction through his stories and poems of mystery. The most outstanding stories by this poet are the black cat, the cask of Amontillado, William Wilson and the man of the crowd. These four stories are the area of interest in this paper and a comparison in the themes of the four stories will be made. DISCUSSION THE STORIES 1.  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   BLACK CAT This story is narrated by Edgar himself and he explains that the story is inspired by his childhood passion for domestic animals. The story is about him and his favorite pet, a cat named as Pluto. Pluto and Edgar were good friends until Edgar changed abruptly, became violent and a drunkard. The situation worsened and Edgar killed the cat, an act that haunted him and prompted him to look for another cat to replace Pluto with. The new black cat does not bring peace to Edgar and the story ends with Edgar killing both the black cat and his wife. The narrator takes the audience through the story with lots of symbolism and imagery. The main theme of this story is the man’s fear of the unknown such as the fear of darkness, illusion and spaces. In the story, Edgar finds his house on fire after killing Pluto, and later he sees an image of a huge cat on the wall of his house. Perverseness is also a theme in this story where the quilt feeling by Edgar causes fear in his life (Barger and Poe, 59). There is also the theme of self destruction which is shown by the way Edgar brings trouble to himself through alcoholism and his violent actions. Another theme is the theme of vengeance (Lippmann 45). The black cat haunts Elgar in order to avenge its death and in the end of the story, the cat alerts draws the attention of the police to the acts of Elgar. The most outstanding elements in this story are the elements of horror and superstition. It is superstition that makes Edgar think that he has seen a cat on the wall of his burnt house, a symbol of Pluto hanging on the noose. Another important element in the story is the cat itself, which plays the roles of an actor, symbol, and a device to develop the plot. It doubles up as both a real creature and a ghost by dying and another cat reappearing in the story. This element of doubling is used in German folklore to signify bad luck or bad omen. The name Pluto is also symbolic as according to Roman mythology, the name symbolizes death or misfortunes. The story is also ambiguous, with the question of whether the reappearance of the cat was natural or superstitious being left unanswered. 2.  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   CASK OF AMONTILLADO In this story the narrator, Montressor, is not happy as the others during the annual celebration of the beginning of Lentern season. The reason why he is not happy is because of the many offenses committed against him by Furtunato. Montressor is out to revenge and he looks for an opportunity to do so. He uses a new wine by the name of Amontillado as bait to catch Furtunato and he succeeds and kills him brutally. The themes in the story are revenge, deception and pride. Montressor is out to revenge against Fortunato for the many offences he has committed, the most recent one being an insult. Deception is another theme shown by the way the way Montressor uses Amontillado to lure Fortunato to the catacombs where he kills him. Pride is evidenced by the believe Fortunato has on his ability to know whether the wine presented to him is truly Amontillado. He believed in his ability even though he was not sober and he was also unwell (Barger and Poe 88). 3.  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   WILLIAM WILSON The story is narrated by a person on the verge of death, and it first builds on flash back. He is disturbed and seeks to get the reader to sympathize with him by informing the reader that his bad deeds were out of unavoidable circumstances. One theme of the story is the enemy within, which means destroying oneself or working against ones interests through alcoholism, drug abuse and others. There is an inner spirit that develops within us and cause fear of some things, depression or lack of sleep. According to Barger and Poe, the narrator is facing such a situation and in his struggle to overcome it, he fights against himself and loses (620). The other theme is the theme of terror which is shown by the way the narrator struggles to fight against an imaginary enemy. 4.  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   THE MAN OF THE CROWD This is the story of a man in the streets of London who has been suffering from an unknown illness. His sits outside and starts categorizing passers by where he notices one old man and follows him. The pursuit extends to the next day and the man is not able to understand the mission of the old man. The main theme in this story is the theme of mystery which is shown in the way the young man can not categorize the old man from the looks, as opposed to the other people in the streets. Even after following the old man for a long time, the man can not tell the motives of the old man and leaves the reader to find out for themselves (Barger and Poe 298). COMPARISON OF THE STORIES   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   Through out the four stories, there are some themes that seem to recur, which are insanity as opposed to rationality, obsession, man and death, double identity, love or hatred, curiosity, resolve among others. About insanity and rationality, in the four stories there are people whose decisions are not driven by rationality but by an element of insanity. In the Black cat, the insanity was brought by influence from alcohol and Elgar is not in control of his decisions (Lippmann 68). Also, in the Cask of Amontillado, Fortunato is driven by drunkenness to accept the challenge to test the wine. The same case applies to the story William Wilson who is so disturbed that his decisions are not rational. In the man of the crowd, the man is driven by an unnamed sickness to sit idly in a coffee shop and categorize people. His move to follow an old man for so long is also an insane decision since he does not stand to benefit from discovering who the old man was.   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   Another common theme in the stories is obsession. The characters in the stories are obsessed with different things and this obsession is a source of misfortunes or death to the characters. In the Black cat, the narrator is obsessed with the cat, his favorite pet and alcohol while in the story of the cask of Amontillado; Fortunato is obsessed with alcohol (Lippmann 176). There is also the obsession with discovering the truth in the man of the crowd. In the story of William Wilson, the narrator is obsessed with fighting against self and wining.   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   Throughout the stories, double identity is used to develop the plot. In the black cat, the black cat doubles as a both a natural creature as well as a ghost by dying and reappearing. In the story of the cask of Amontillado, the wine doubles as something to cheer up Fortunato and also as something to kill him. In the story William Wilson, the narrator’s struggle against self results to both a win and a loss while in the story the man of the crowd, the young man doubles as the man we know as well as a mysterious man.   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   In the stories, Elgar has employed the use of symbolism, anaphora and first person narration in his work. In the story of the black cat, the cat is used to symbolize misfortune. In the cask of Amontillado, the name ‘Amontillado’ is also used to show bad luck. Anaphora is repeating a phrase or a word before the beginning of lines often to show emphasis and create balance. In the story of the black cat, he uses anaphora as shown in the line â€Å"...I blush, I burn, I shudder, while I pen†¦..†(Barger and Poe 62) and in the story of William Wilson, anaphora is shown in the line â€Å"†¦The same name! The same contour of person! The same of arrival...† (Barger and Poe 625).Irony is also used as shown in the story of the black cat. The cat which was initially a great friend to Edgar turned to be an enemy and cause of trouble to him. In the cask of Amontillado, when Montressor gives Fortunato wine, he toasts â€Å"to his long life† knowing very well that h would kill him. When they are ascending the stairs, he also tells him that his life is respected, which was not true since he was planning to kill him.  Ã‚   CONCLUSION Allan Edgar was a good writer who used various tools in his short stories to develop the story as well as to bring out the various themes. His favorite themes were mystery, obsession and revenge and these are common in most of his works (Hossick 67). He used the first person narration since it is more captivating and also convenient. His short stories are informative, thrilling as well as entertaining due to his literature skills.    WORKS CITED Barger, Andrew and Poe, Allen, E. Edgar Allen Poe Annotated and illustrated entire Stories and Poems. London: Bottletree Books LLC, 2008. Print. Hossick, Malcolm. Edgar Allen Poe. New York: Ardent Media, 2007. Print Lippmann, Babette. Edgar Allen Poe- â€Å"The Philosophy of Composition†: An Analysis of His Work. Norderstedt: GRIN Verlag, 2007.Print

Finance and Accounting Individual Assignment Essay

Finance and Accounting Individual Assignment - Essay Example It was later reorganized in 2001 to become the International Accounting Standards Board (IASB). In 1973 the Financial Accounting Standards Board (FASB) was also born. The accounting standards that the FASB helps formulate for accountants in the United States are the generally accepted accounting principles (GAAP). Over 100 countries around the world are currently using the international financial reporting standards (IFRS) created by the IASB. The amount of countries that have adopted the IFRS has drastically increase in the 21st century since prior to 2002 only eight countries were using IFRS (Fasb, 2011). In the 1990s the concept of accounting convergence began to raise interest among the business community which caught the attention of FASB and IASC. In 1994 the FASB and IASC undertook their first standard setting project together. The purpose of the project was to improve the earnings per share (EPS) standards to eliminate any differences in the calculation of the metric between the two standard setting bodies. The following year both standard setting bodies collaborated in another project whose purpose was to compare the U.S. GAAP and the IASC standards. Once the IASC was converted into the IASB in 2001 the pace of convergence accelerated a lot. One of the major events that helped the IASB gain credibility worldwide was the adoption of the European Union of IFRS. A project that was signed in 2002 in which the FASB and IASB agreed to improve convergence between U.S. GAAP and IFRS was the Norwalk Agreement. â€Å"The Norwalk Agreement set out the shared goal of developing compatible, high-quality accounting standards that could be used for both domestic and cross-border financial reporting† (Fasb, 2011). In 2006 the FASB and IASB issues a Memorandum of Understanding that created a two year deadline to achieve greater progress in the convergence project by 2008. In 2007 the Securities and Exchange Commission released a concept released that allowed com panies the optional use of IFRS by U.S issuers in the financial statements filed by companies for the SEC. Another sign of progress in the convergence project was the creation in 2007 of a converged standard by the FASB and IASB for business combinations. In 2008 the SEC issued a proposed roadmap to adoption of IFRS in the United States and a proposed rule on optional early use of IFRS. The latest update on the convergence project was issued on April 21, 2011 in a document called Progress Report on IASB-FASB Convergence Work (Fasb, 2011). The MSA program is a great opportunity for me to develop my skills and abilities in the accounting profession. In the competitive work environment of the 21st century gaining a graduate education can give me a competitive advantage over other candidates. In the United States only about 6.8% of the population has a master’s degree (Info, 2005). The five courses accounting courses in the UOP MSA curriculum look like outstanding courses that wi ll help me gain more specialized knowledge in accounting. I am looking very forward to taking the forensic accounting course. â€Å"Forensic accounting is a specialty requiring the integration of investigative, accounting, and auditing skills† (Answers, 2011). Forensic accounting can be used by corporations to solve legal problems. Once I complete this program the doors of opportunity are going to open for me. Accounting is one of the hottest professional fields. Due to the complexity

Protecting Information Resources Assignment Example | Topics and Well Written Essays - 250 words

Protecting Information Resources - Assignment Example Instead, one should memorize one’s PIN to prevent an identity thief from determining it. A third recommendation is analyzing one’s credit releases. To know about possible identity thefts, one should go through his or her credit reports regularly to view transactions, registrations, approvals, and any previous activity on one’s cards or accounts. Regular analysis hinders potential harm since one detects identity theft promptly. Fourthly, shredding documents with one’s personal information or numbers is a critical and safe way of disposing it. A shredding machine is the simplest, relatively cheap, and best way to safeguard oneself from dumpster divers. One hinders identity thieves from getting personal information from one’s trash by shredding document such as preapproved cards, checks, bank declarations, and deposit slips. Lastly, checking one’s mail early lowers exposure to mail theft. One can ask the post office to hold your mail when one has traveled for a given period. Also locking the mailbox prevents thieves from accessing mail with personal

HIV (Human Immunodeficiency Virus) Essay Example for Free

HIV (Human Immunodeficiency Virus) Essay HIV (Human Immunodeficiency Virus) causes AIDS (Acquired Immune Deficiency Syndrome) that disables the immune system.  It was discovered in 1983 .HIV enters the body through the bloodstream and duplicates itself rapidly.  The victim is susceptible to infectious diseases that eventually are fatal (1). Statistically, HIV/AIDS is the number one killer of African-American women ages 25 to 34, according to the Centers for Disease Control and Prevention.  Between 2000 and 2003, they were nineteen times more likely than White females and five times more likely than Hispanic females to contract the disease.   African-American men were seven times more likely than White men and three times the rate of Hispanic men to contract HIV/AIDS (2). In 2008, there is still not a cure for AIDS.  Instead, scientists have discovered drugs that can slow down the progression of the disease. Protease inhibitors (PIs) are antiviral drugs that slow down the spread of HIV (1). The virus produces a protein called protease so that it can replicate itself.  Ã¢â‚¬Å"Protease cuts long chains of proteins and enzymes into shorter chains, the first step in the process by which HIV infects a cell† (1).  If this doesn’t happen, replication does not continue. In 1987, AZT was the first anti-HIV medication that was created.   It showed hope and kept people healthier longer.   However, the side effects were very high for men.   Since that time, more HIV medications have evolved.   Antiretroviral therapy usually consists of combinations of nucleoside/nucleotide reverse transcriptase inhibitors, non-nucleoside reverse transcriptase inhibitors, protease inhibitors, or fusion inhibitors, which are prescribed in the later stages of HIV (19). Dual protease inhibitor therapy is also being used clinically (3).  They are also associated with improving morbidity and mortality of HIV-positive persons (5).  Although these drugs are expensive, they have proven to be the most successful therapy in managing HIV.   However, some patients don’t comply 100% with their treatments.   This is largely due to the side effects. Sometimes they make a patient feel worse than the actual disease. The most commonly reported ones are abdominal pain, abnormal bowel movements, diarrhea, fatigue, headache, and nausea.   Children usually develop a skin rash. The more serious side effects are liver problems and pancreatitis.   Some patients have also seen large increase in triglyceride ad cholesterol levels.   Diabetics saw an increase in their blood sugar levels.   Other patients developed diabetes while taking protease inhibitors (16). Presently, nine PIs have been approved for use in the United States and Europe:   amprenavir, atazanavir, fosamprenavir, lopinavir, indinavir, ritonavir, saquinavir, tipranavir, and nelfinavir. Three non-nucleoside reverse transcriptase inhibitors (NNRTIs) are used for treatment of HIV:  nevirapine, delavirdine, and efavirenz (7). Drug treatment selection depends on factors such as drug resistance, tolerability, drug interactions, and effectiveness. Therapeutic drug monitoring (TDM) â€Å"has been proposed so that practitioners may better maintain appropriate plasma concentrations of drugs in their patients by identifying interactions with other medications and assessing medication adherence   (7). Figure 1 shows the structures of these compounds. Fig. 1. Chemical structures of protease inhibitors and non-nucleoside reverse transcriptase inhibitors. About 50% of â€Å"treatment-naà ¯ve patients† don’t have continued antiviral response after one year of therapy (18). In some cases, there is a development of drug resistance and metabolic complications.   Also, â€Å"there is increasing evidence that virological treatment failure is correlated with variations in the pharmacokinetic parameters of drugs (20).   This can be due to drug interactions, low bioavailability, and variations in metabolic enzyme activity.   Atazanavir (ATV) has good oral bioavailability and a favorable pharmacokinetics profile (18).   With this in mind, patients can mostly take a once-daily dose. A separate analytical method has been recently published for quantifying ATV in human plasma using solid phase extraction and HPLC with PDA (photodiode array) detection at 201 nm (18).   This method provides excellent separation of ATV from its internal standard, clozapine (CLZ) and the other PIs, thus, obtaining an accurate measurement of the drug (see figure 2). CLZ elutes at 8.9 minutes, and ATV elutes a 24.4 minutes. A 40-ml injection resulted in a recovery yield of 100%. Fig. 2.   Chromatogram of ATV with PIs/NNRTIs (8000 ng/ml) spiked with CLZ Nelfinavir mesylate has been shown in phase III controlled clinical trials to significantly reduce viral load and increase CD4+ cell counts when used with reverse transcriptase inhibitors. It’s prescribed as part of triple drug combination therapy (9). CD4+ are helper T cells.   They are important for immune reconstitution in patients that are receiving antiretroviral therapy (10).   After a large number of these cells are destroyed, AIDS develops (7). Six clinical trials was conducted in the past ten years using 2, 148 HIV-infected children enrolled in the Pediatric AIDS Clinical Trials Group treatment trials (10).   Patient ages varied from 2 to 7 years of age.   The focus was to observe short-term variability of CD4 percentages.   The study found that 49% of patients had CD4 percentages above 25%.   32% of patients had CD4 counts between 15% and 24%; 19% were less than 15%.   Finally, 5.4% had a CD4% of less than 5% (10). In June 2006, Darunavir (DRV) was licensed in the United States.   It is a promising PI that is active against HIV strains that are resistant to the other PIs that currently on the market (17).   It is prescribed in 600-mg doses that are taken twice daily with 100 mg of ritonavir that acts as a booster. Protease inhibitors are associated with unfavorable pharmacokinetics and many side effects such as gastrointestinal disturbances and lipid abnormalities (5).  Four of the most common PIs used are indinavir, ritonavir, saquinavir, and nelfinavir (3).  Ã¢â‚¬Å"Monitoring blood concentration of PIs, which can indicate both therapeutic and toxic levels of the drugs as well as patient noncompliance with the medication, may improve the care of both HIV-infected adults and children† (3). When ritonavir was first introduced, it was given in doses of 600 mg every 12 hours. â€Å"However, patient intolerance of full doses led to its primary use as a pharmacologic enhancer to increase the concentrations in plasma of a second protease inhibitor to improve the convenience of antiretroviral regimens by extending the dosing interval, reducing pill burden, and /or eliminating food-induced reductions in pharmacokinetic exposure† (8).   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Researchers used High Performance Liquid Chromatography (HPLC) to determine concentrations of PIs in blood.  Various methods have been used to study the pharmacokinetics and drug interactions. Interpreting plasma levels can be used to â€Å"individualize drug dosage of antiretrovirals (4). Quality control (QC) procedures must be done to ensure that these methods are accurate and precise.  Ã¢â‚¬Å"Such procedures usually include intralaboratory (internal) method validation, intralaboratory QC procedures, and participation in an interlaboratory QC program for antiretroviral drugs† (4). Since the latter hadn’t been done before, it was established so that laboratories can obtain better measurement results of antiretroviral drugs. Nine laboratories participated in the first part of the program.  The first part of the experiment involved the measurement of the protease inhibitors:  indinavir, nelfinavir, ritonavir, and saquinavir.  All had a specified purity of 99% or higher.  QC samples were prepared by spiking blank plasma from HIV-negative volunteers with PI standard. The low concentrated standards ranged from 0.087 to 0.15 mg/L while the intermediate concentrated standards contained ranged from 2 to 3 mg/L of all four PIs.  Finally, the high-concentrated standards contained approximately 5 to 11 mg/L of drug.  All drugs were dissolved in methanol following accurate weighings and diluted with blank plasma (4). High Performance Liquid Chromatography (HPLC) was used to analyze twelve samples.  All laboratories measuring more than one protease inhibitor used as assay for â€Å"simultaneous determination† (4).  Six laboratories used HPLC/UV and three labs used HPLC-MS/MS. Mass spectrometry detection is often recommended for measurement of low concentration levels.  Also, this type of analysis is usually faster and â€Å"does not require complete resolution of drugs for detection and quantification† (5).  Only five laboratories were able to measure all four PIs.  Three laboratories were not able to determine nelfinavir.  One laboratory only measured indinavir (4). Acceptable accuracy results are between 80% and 120%.  Only indinavir resulted in an acceptable accuracy of 80%.  The remaining PIs had between 36% and 74% accuracy.  These results should encourage laboratories to improve their analytical methods and QC procedures.  Other PIs, such as amprenavir and lopinavir, can be analyzed as well (4). HIV-positive plasma samples are heat inactivated before analysis, approximately 58 °C for 40 minutes, to decrease the risk of infection to the operator.  They may also go through a freeze/thaw cycle. As with the QC study, blank plasma was spiked with seven PIs (indinavir, amprenavir, atazanavir, ritonavir, saquinavir, lopinavir, and nelfnavir) at low, intermediate, and high concentrations measured in ng/ml.  Certain assays only require 100  µl of plasma for analysis.  Ã¢â‚¬Å"This is advantageous when measuring PI concentrations as part of clinical studies as they often necessitate hourly sampling to generate complete PK profiles; therefore less blood can be drawn from the patient† (5).  In addition, seven PIs can be quantified in one assay, but impossible to assay all seven in a single preparation. â€Å"The use of liquid chromatography coupled to tandem mass spectrometry (LC-MS-MS) has emerged as the developmental method of choice supporting clinical and pre-clinical pharmacokinetic studies† (13). Recovery for the HPLC-MS/MS methods was above 87% for all seven drugs at all three concentration levels (5).  It was successful in quantifying seven PI concentrations in plasma of HIV positive persons that participated in a run time of nine minutes.  Therefore, the assay may be used for determining PI concentrations in semen, lymphocytes, and cerebrospinal fluid (5). A fast and highly-sensitive LC-MS-MS method was developed that could analyze five protease inhibitors (amprenavir, indinavir, nelfinavir, ritonavir, and saquinavir) in one run using an internal standard.   Sample sizes were small (ng/ml) and run times were approximately 5 minutes.   Recoveries for all five PIs were between 87% and 92%(11). Tipranavir is part of a class of non-peptidic PIs that works against both â€Å"wild-type virus and variants resistant to current PIs†(6).  It also has a high genetic barrier.  Tipranavir is prescribed in a 500-mg dose taken in combination with 200 mg of ritonavir twice daily as part of antiretroviral therapy for patients with HIV-1 strains that are resistant to multiple PIs (6). Fig. 3. Tipranavir chemical structure   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  An HPLC-UV method has been validated and is currently applied when monitoring tipranavir (TPV) plasma levels in HIV patients.  Samples were prepared for solid phase extraction (SPE) by conditioning the cartridges with 0.1% phosphoric acid, pH 7.  TPV stock solutions with a concentration of 5 mg/ml were diluted from with 50% methanol. Samples were spiked with TPV at 1.875, 7.5, 18.75, 37.5, 60, and 75  µg/ml in triplicate. QC samples were diluted with blank plasma and phosphate buffer to 5.625, 22.5, and 67.5  µg/ml.  Clozapine was used as the internal standard (6).  5-ml aliquots of blood samples were obtained from HIV infected patients.  The plasma obtained from centrifugation was heated at 60 ° C for one hour in a water bath. TPV in plasma was measured at a UV absorbance of 201 nm with a retention time of 32.2 minutes. Its internal standard, clozapine (CLZ) has a retention time of 8.3 minutes (6). Figure 4 below shows the chromatogram of TPV, its internal standard, and all other PIs and NNRTIs  present.   Figure 5 shows TPV and its internal standard CLZ only. Fig. 4. Chromatogram of plasma control sample of TVP (22.5 mg/ml) spiked with internal standard and all PIs and NNRTIs. Fig. 5. Chromatogram of calibration sample of TVP (37.5 mg/ml) spiked with internal standard CLZ. Isocratic HPLC methods combined with the use of UV and fluorescence detection produces more sensitivity. Amprenavir is a fluorescent compound.   Its internal standard PR25 can also be seen under fluorescence (see figure 6). Fig. 6.   Blank plasma with 100 ng/ml amprenavir and 1000 ng/ml of PR25 seen under fluorescence. In conclusion, much progress has been made in the development of protease inhibitors and other antiretroviral therapy. HPLC with UV detection has been the most commonly used method of analysis.   It is rapid, simple, and highly sensitive.   LC-MS-MS has been noted at the developmental method of choice for clinical and pre-clinical pharmacokinetic studies (13).   More compounds can be analyzed in less time.   Also, they can be used for methods using other human biological matrices. For the majority of protease inhibitors, all side effects are not known. In 2007, darunavir was the new HIV protease inhibitor with eleven other antiretroviral agents on the market.   They can affect patients that have diabetes, liver problems, and hemophilia; their conditions can worsen as a result of taking PIs. Only a patient’s health care provider can determine the best treatment option REFERENCES Nagel, Rob. 2007. Protease Inhibitors. UXL Encyclopedia of Science; [cited 2008 May 8], Available from http://galenet.galegroup.com/servlet/SciRC?ste=1docnum=cv2644301082. Health Hotline:   HIV and Genital Herpes [cited 2008 May8]; Available from http://www.ebony.com. â€Å"HPLC Assay for Common Protease Inhibitors Developed.†Antiviral Weekly; [cited 2008 May 07; Available from http://galenet.galegroup.com/servlet/SciRC?ste=1docNum=A60069570. Aarnoutse, Rob E., Verweij-van Wissen, Corrien P.W.G.M., van Ewijk-Beneken Kolmer, Eleonora, W.J., Wuis, Eveline, W., Koopmans, Peter P., Hekster, Yechiel A., and Burger, David, M. 2001. International Interlaboratory Quality Control Program for Measurement of Antiretroviral Drugs in Plasma; Antimicrobial Agents and Chemo 46(3): 884-886. Dickinson, Laura, Robinson, Lesley, Tjia, John, Khoo, and Saye, Back, David. 2005. Simultaneous determination of HIV protease inhibitors amprenavir, atazanavir, indinavir, lopinavir, nelfinavir, ritonavir and saquinavir in human plasma by high-performance liquid chromatography-tandem mass spectrometry; J of Chrom. B 829:82-90. Colombo, S., Beguin, A., Marzolini, C., Telenti, A., Biollaz, J., and Decosterd, L.A. 2006.   Determination of the novel non-peptidic HIV-protease inhibitor tipranavir by HPLC-UV after solid-phase extraction; J of Chrom. B 832:138-143. Rezk, Naser L., Tidwell, Richard R., and Kashuba, Angela D.M. 2004.   High-performance liquid chromatography assay for the quantification of HIV protease inhibitors and non-nucleoside reverse transcriptase inhibitors in human plasma; J of Chrom. B 805:241-247. Shelton, Mark J.,Hewitt, Ross G., Adams, John, Dela-Coletta, Andrew, Cox, Steven, and Morse, Gene D. 2003. Pharmacokinetics of Ritonavir and Delavirdine in Human Immunodeficiency Virus-Infected Patients; Antimicrobial Agents and Chemo 47(5): 1694-1699. Zhang, Kanyin E., Wu, Ellen, Patick, Amy K., Kerr, Bradley, Zorbas, Mark, Lankford, Angela, Kobayashi, Takuo, Maeda, Yuki, Shetty, Bhasker, and Webber, Stephanie. 2001.Circulating Metabolites of the Human Immunodeficiency Virus protease Inhibitor Nelfinavir in Humans:   Structural Identification, Levels in Plasma, and Antiviral Activities; Antimicrobial Agents and Chemo 45(4): 1086-1093. Carey, Vincent J., Pahwa, Savita, and Weinberg, Adriana. 2005.   Reliability of CD4 Quantitation in Human Immunodeficiency Virus-Positive Children:   Implications for Definition of Immunologic Response to Highly Active Antiretroviral Therapy; Clinical and Diagnostic Lab Immunology 12(5): 640-643. Chi, Jingduan, Jayewardene, Anura L., Stone, Judith A., Motoya, Toshiro, and Aweeka, Francesca. 2002.   Simultaneous determination of five HIV protease inhibitors nelfinavir, indinavir, ritonavir, saquinavir and amprenavir in human plasma by LC/MS/MS; J of Pharm and Biomedical Analysis 30:675-684. Verbesselt, R., Van Wijngaerden, E., and de Hoon, J. 2007. Simultaneous determination of 8 HIV protease inhibitors in human plasma by isocratic high-performance liquid chromatography with combined use of UV and fluorescence detection: Amprenavir, indinavir, atazanavir, ritonavir, lopinavir, saquinavir, nelfinavir and M8-nelfinavir metabolite; J of Chrom. B 845:51-60. Frerichs, Valerie A., DiFrancesco, Robin, and Morse, Gene D. 2003. Determination of protease inhibitors using liquid chromatography-tandem mass spectrometry; J of Chrom, B 787:393-403. Gangl, Eric, Utkin, Ilya, Gerber, Nicholas, and Vouros, Paul. 2002. Structural elucidation of metabolites of ritonavir and indinavir by liquid chromatography-mass spectrometry; J. of Chrom. A 974:91-101. Turner, Michele L., Reed-Walker, Kedria, King, Jennifer R., and Acosta, Edward P. 2003. Simultaneous determination of nine antiretroviral compounds in human plasma using liquid chromatography; J. of Chrom. B 784:331-341. 2008. Kaletra. [Cited 2008 May 16], Available from http://www.heartandsoul.com. D’Avolio, Antonio, Siccardi, Marco, Sciandra, Mauro, Lorena, Baietto, Bonora, Stefano, Trentini, Laura, and Di Perri, Giovanni.2007. HPLC-MS method for the simultaneous quantification of the new HIV protease inhibitor darunavir, and 11 other antiretroviral agents in plasma of HIV-infected patients; J. of Chrom. B 859:234-240. Colombo, S., Guignard, N., Marzolini, C., Telenti, A., Biollaz, J., and Decosterd, L.A. 2004. Determination of the new HIV-protease inhibitor atazanavir by liquid chromatography after solid-phase extraction; J. of Chrom. B 810:25-34. Weller, Dennis R., Brundage, Richard C., Balfour, Jr., Henry H., and Vezina, Heather E. An isocratic liquid chromatography method for determining HIV non-nucleoside reverse transcriptase inhibitor and protease inhibitor concentrations in human plasma; J. of Chrom. B 848:369-373. Sarasa-Nacenta, Maria, Lopez-Pua, Yolanda, Mallolas, Josep, Blanco, Jose’ Luis, Gatell, Jose’ M., and Carne’, Xavier. Simultaneous determination of the HIV-protease inhibitors indinavir, amprenavir, ritonavir, saquinavir and nelfinavir in human plasma by reversed-phase high- performance liquid chromatography; J, of Chrom. B 757:325-332.

Quest for Eternity in the Poetry of Dickinson Essay -- Biography Biogr

Quest for Eternity in the Poetry of Dickinson      Ã‚  Ã‚   Over the past few decades, a considerable number of comments have been made on the idea of eternity in Emily Dickinson's poetry. The following are several examples: Robert Weisbuch's Emily Dickinson's Poetry (1975), Jane Donahue Eberwein's Dickinson: Strategies of Limitation (1985), Dorothy Huff Oberhaus' Emily Dickinson's Fascicles: Method and Meaning (1995), and James McIntosh's Nimble Believing: Dickinson and the Unknown (2000). However, opinions vary as to how Dickinson explored the question regarding eternity; much ink has still been spent on the issue. This paper, therefore, provides another discussion of the idea of eternity depicted in Dickinson's poetry. I will discuss the issue by considering how her poems describe the process through which the poet finally reaches the belief in eternity-overcoming the feud between Christianity and scientific knowledge and that between Romanticism and existentialism.    As a beginning, let us look closely at one of the poems in which Dickinson gives a detailed account of a deathbed scene: The last Night that She lived It was a Common Night Except the Dying-this to Us Made Nature different We noticed smallest things- Things overlooked before By this great light upon our Minds Italicized-as 'twere. As We went out and in Between Her final Room And Rooms where Those to be alive Tomorrow were, a Blame That Others could exist While She must finish quite A Jealousy for Her arose So nearly infinite-- (P-1100)    It is presumed that Dickinson wrote this piece of verse in circa 1886. In May of that year, Laura Dickey, the wife of Frank W. of Michigan, ... ...Dickinson. 2 vols. 1974. Cambridge: Harvard UP, 1994. Stocks, Kenneth. Emily Dickinson and the Modern Consciousness. Hong Kong: Macmillan, 1988. Wolff, Cynthia Griffin. Emily Dickinson. 1986. Reading: Addison, 1988. Works Consulted Kjaer, Niels Pastor. "The Poet of Moment: Emily Dickinson and Soren Kierkegaard." Dickinson Studies 59 (1986): 46-9. McIntosh, James. Nimble Believing: Dickinson and the Unknown. Ann Arbor: U of Michigan P, 2000. Oberhaus, Dorothy Huff. Emily Dickinson's Fascicles: Method and Meaning. University Park: Pennsylvania State UP, 1995. Rosenbaum, S. P., ed. A Concordance to the Poems of Emily Dickinson. Ithaca: Cornell UP, 1964. Scholnick, Robert J., ed. American Literature and Science. Lexington: UP of Kentucky, 1992. Weisbuch, Robert. Emily Dickinson's Poetry. Chicago: Chicago UP, 1975.   

Pathogenic Parasite and Waterborne Diseases in Three Stations

Chapter 1 INTRODUCTION Background of the Study Rivers are the bodies of water bearing an immense biological importance. Though they contain only about 0. 0001% of the total amount of water in the world at any given time, rivers are vital carriers of water and nutrients to areas all around the earth, they are essential components of the hydrological cycle, for they act as drainage channels for surface water and they serve as habitat for various organisms (Hebert, 2011). With rapid increase of the country’s population and urbanization, bodies of water tremendously began to be polluted.This event commenced the contamination of drinking water as well as the widespread occurrence of waterborne diseases. Waterborne diseases are often caused by parasites which are directly transmitted through consuming contaminated drinking water. Any water reserve, infested with pathogenic parasites, used in the preparation of food can be considered as a source of foodborne disease and could be easi ly transmitted through consumption of the same pathogenic parasites. These diseases commonly affect the digestive tract as well as the other vital parts of the body that may be fatal to anyone especially those who are immunocompromised (WHO, 2004).Globally, waterborne diseases such as diarrhea accounts for 1. 5 million deaths annually according to the Centers for Disease Control and Prevention on its report in 2010. It is considered one of the most dangerous diseases that could be acquired in flowing water and is a major health problem for developing countries. Also, according to World Health Organization (WHO) on its report in 2004, diarrheal disease accounts for an estimated 4. 1% of the total Disability-adjusted Life Year (DALY) global burden of disease and is responsible for the deaths of 1. million people every year. It was estimated that 88% of that burden is attributable to unsafe water supply, sanitation and hygiene, and is mostly concentrated in children in developing count ries. Over the time – from 2004 up to the present – , rapid increase in population, urbanization, and industrialization reduce the quality of Philippine waters, especially in densely populated areas and regions of industrial and agricultural activities. The discharge of domestic and industrial wastewater and agricultural runoff has caused extensive pollution of the receiving ater-bodies. This effluent is in the form of raw sewage, detergents, fertilizer, heavy metals, chemical products, oils, and even solid waste. Each of these pollutants has a different noxious effect that influences human livelihood and translates into economic costs. Access to clean and adequate water remains an acute seasonal problem in urban and coastal areas in the Philippines. The National Capital Region (Metro Manila), Central Luzon, Southern Tagalog, and Central Visayas are the four urban critical regions in terms of water quality and quantity.The Government’s monitoring data indicates that just over a third or 36 percent of the country’s river systems are classified as sources of public water supply, up to 58 percent of groundwater sampled is contaminated with coliform and needs treatment, approximately 31 percent of illness monitored for a five-year period were caused by waterborne sources, and many areas are experiencing a shortage of water supply during the dry season. Nearly 2. 2 million metric tons of organic pollution are produced annually by domestic (48 percent), agricultural (37 percent), and industrial (15 percent) sectors.In the four water-critical regions, water pollution is dominated by domestic and industrial sources. Untreated wastewater affects health by spreading disease-causing parasites, makes water unfit for drinking and recreational use, threatens biodiversity, and deteriorates overall quality of life. Known diseases caused by poor water include gastro-enteritis, diarrhea, typhoid, cholera, dysentery and hepatitis. The number of water- related health outbreaks including deaths reported in newspapers is going up.However, awareness regarding the need for improved sanitation and water pollution control, reflected by the willingness-to-pay and connection to a sewerage system where they are easily available, is very low (Philippines: Environment Monitor, 2003; Greenpeace 2007). Davao City has been expanding and growing in terms of population, economy and industry. In this manner, more raw materials are being demanded by these different sectors and as a result destruction of the natural resources is inevitable. Davao river ranks number seven among the largest river basins in the Philippines.This is largest of the city’s nine principal watersheds. The river is the main natural reservoir of the aquifer in the city’s jurisdiction. Its length is about 143 kilometers. It has received domestic and industrial wastewater. Since the sewage systems have not yet been fully developed, the garbage has been discharge in to the river leading to a wide-scale contamination of Davao River (Pascua et. al. , 2011). At present, residents within Davao River unceasingly make use of the water for bathing and washing which may present various threats to them.Some use the river for livelihood e. g. sand quarrying and fishing and for recreational activities. But they are blinded by the fact that they can be infected by pathogenic parasites that would eventually lead to waterborne diseases. This study aims to determine the level of pathogenic parasites in the water. Having everyone in the community know the specific parasites present in the selected stations of Davao River – Bankerohan, Bucana and Magallanes – would eventually lead to a more effective prevention and disease control among the residents of the community.The goal of clean water for everyone has to be achieved in a growing population of a city. This study also aims to measure the level of waterborne diseases in Davao River. Through thi s study, we can help the people be oriented with the danger of using contaminated water from Davao River. Related Literature The importance of urban rivers and streams for urban ecology and quality of life in cities is an increasing concern worldwide. The present state of rivers is much dependent on the history and trends of the society in each country.In many situations water quality is still unsatisfying. Restoration of urban rivers is challenging because of intensive land use, danger of flooding and impacts of human activities for water quality and for the ecology of rivers (Jormola, 2008). Today about 50% of the global population are living in urban areas, placing one-third of their inhabitants into slums, and creating huge challenges to their environment and sanitation. Poor sanitation, poor treatments of waste water, as well as catastrophic floods introduce pathogenic bacteria into rivers, infecting and killing many people.The goal of clean water for everyone has to be achieve d with a still growing human population and their rapid concentration in large cities, often megacities. How long introduced pathogens survive in rivers and what their niches are remain poorly known but essential to control waterborne diseases in megacities. Biofilms are often niches for various pathogens because they possess high resistances against environmental stress. They also facilitate gene transfers of antibiotic resistance genes which become an increasing health problem.Beside biofilms, amoebae are carriers of pathogenic bacteria and niches for their survival (Abraham, 2011). The World Health Organization says that every year more than 3. 4 million people die as a result of water related diseases, making it the leading cause of disease and death around the world. Most of the victims are young children, the vast majority of whom die of illnesses caused by organisms that thrive in water sources contaminated by raw sewage (Berman, 2009). Waterborne Diseases Many illnesses, con taminants, and injuries can be water, sanitation, or hygiene-related.Waterborne diseases are caused by organisms that are directly spread through water. Water-related illnesses can be acquired due to lack of water for good hygiene and lack of sanitation. It is important to know how these diseases and contaminants affect humans where they are found, and how to reduce the chance getting ill or suffering injury. This will allow individuals to make informed decisions about water, hygiene, and sanitation-related activities (CDC, 2012). Parasitic Organisms Parasites may be present in food or in water and can be identified as causes of waterborne illness.They range in size, from tiny single-celled organisms to worms visible to the naked eye. Their lifecycle may also vary. While some parasites use a permanent host, others go through a series of developmental phases using different animal or human hosts. The illnesses they can cause range from mild discomfort to debilitating illness and poss ibly death (USDA, 2011). Parasites cause over 2 billion infections per year worldwide. In the immunocompromised host these infections generally represent reactivation of infection from donor or in the allograft recipient often long after the initial infection (Wiley, 2004).Inland and coastal surface waters can be contaminated by human waterborne zoonotic enteropathogens such as Cryptosporidium parvum, Giardia lamblia, Encephalitozoon intestinalis, E. hellem and Enterocytozoon bieneusi (Graczyket et. al, 2008). The aquatic ecosystem harbors many kinds of organisms. Some of these organisms are parasitic protozoa such as Cryptosporidium spp. and Giardia spp. , which have recently been recognized as important causes of water and food-borne disease outbreaks associated with fecal contamination (Doron, 2000; Karanis et al. , 2006).Cryptosporidium is a parasite commonly found in lakes and rivers, especially when the water is contaminated with sewage and animal wastes. There have been six m ajor outbreaks of cryptosporidiosis in the United States as a result of contamination of drinking water. One major outbreak in Milwaukee in 1993 affected over 400,000 persons. Cryptosporidial infection can thus be transmitted from fecally contaminated food and water, from animal-person contact, and via person-person contact. The probability of transmission from just a small amount of contamination is fairly high, since a recent study has determined that the 50% infective dose of C. arvum is only 132 oocysts for healthy persons with no previous serological immunity to cryptosporidiosis (Hannahs, G. 1995). Cryptosporidium is very resistant to disinfection, and even a well-operated water treatment system cannot ensure that drinking water will be completely free of this parasite. Cryptosporidium has caused several large waterborne disease outbreaks of gastrointestinal illness, with symptoms that include diarrhea, nausea, and/or stomach cramps (Freedrinkingwater. com, 2009). Cryptosporid iosis, a common finding in pediatric patients hospitalized for diarrhea in urban Manila, Republic of the Philippines.Urban areas, particularly in the developing countries, frequently experience severe overcrowding with concomitant diminished sanitation. This situation is conducive to the transmission of enteric pathogens. It would be reasonable to expect that cryptosporidium oocysts are readily passed from human-to-human, human-to-animal and animal-to-human hosts. In rural areas around the world, human cryptosporidiosis is putatively associated with close human-animal contact, as in the case of animal handlers and similar agricultural situations (Laxer, M. 2003).Giardia lamblia is the protozoan parasite responsible for the disease Giardiasis. Symptoms of acute Giardiasis include diarrhea, nausea, weight loss, malabsorption, abdominal cramps, flatulence, and anemia. The mode of transmission of Giardia is through fecal to oral route or ingestion of cysts. A person can become infected after accidentally swallowing the parasite; but cannot become infected through contact with blood. You can become infected with Giardia lamblia if you swallow contaminated recreational water. Recreational water such as in swimming pools, hot tubs, jacuzzis, fountains, lakes, rivers, prings, ponds, or streams that can be contaminated with sewage or feces from humans or animals (Vdresearch. com, 2008). The prevalence of Giardia and Cryptosporidium among 3,456 diarrheic patients corrected from May 2004 to May 2005 in the Philippines was determined. Of 133 (3. 8%) positive samples, 69 (2. 0%) were positive for Giardia and 67 (1. 9%) for Cryptosporidium. Three samples had co-infection with Giardia and Cryptosporidium. Luzon had the highest positive samples (5. 0%) followed by Mindanao (4. 9%), then Visayas (2. 2%). Giardia was most prevalent in Mindanao (3. 6%) while Cryptosporidium was most prevalent in Luzon (3. %). The prevalence of Giardia (2. 0%) among pediatric patients (0-18 years ) did not significantly differ from that (1. 9%) among adults (>18 years old). However, for Cryptosporidium, the prevalence (2. 9%) among pediatric patients was significantly higher compared to that (0. 2%) among adult patients. In the pediatric population, the highest percentage of patients with Giardia was the 5-9 year old age group, while that of Cryptosporidium was in the 0-4 year old group. The prevalence of Giardia, but not Cryptosporidium, was significantly higher in male than female adults (Natividad, F. 008). Amoebic dysentery (amoebiasis) which is the agent of Entamoeba histolytica is widely seen around the world. About 50 million people has become infected a year and eventually over 100,000 people lose their lives. Amoebiasis come into being in consequence of taken of quad-core mature cysts from water, foods, goods or hands by orally. E. histolytica trophozoites are placed into the colon mucosa and submucosa then forms a bloodymucus diarrhea table. It forms abscesses by m oving through blood to liver, lungs, brain and other tissues (Kaya, O. 2011).The infection is common in developing countries and predominantly affects individuals with poor socioeconomic conditions, nonhygienic practices, and malnutrition (Rivera, W. 1998). Related Studies Waterborne diseases are the most prevalent infectious diseases in the developing countries especially in new settlements along the river. Waterborne diseases occur worldwide, and outbreaks caused by the contamination of community water systems and accidental ingestion of recreational waters have the potential to cause disease in large numbers of consumers. Statistics on outbreaks linked to contaminated water have been vailable in the USA since 1920 (Craun 1986), and since 1971, the Centers for Disease Control (CDC), the US Environmental Protection Agency (USEPA), and the Council of State and Territorial Epidemiologists have maintained a collaborative surveillance system for collecting data pertaining to the occurr ence and causes of outbreaks of waterborne disease (Barwick et al. 2000; Lee et al. 2002). In Europe during 1986–96, 277 outbreaks associated with drinking and recreational water were reported from 16 European countries (Kramer et al. 2001). At least 325 water-associated outbreaks of parasitic protozoan disease have been reported.North American and European outbreaks accounted for 93% of all reports and nearly two-thirds of outbreaks occurred in North America. Over 30% of all outbreaks were documented from Europe, with the UK accounting for 24% of outbreaks, worldwide. Giardia duodenalis and Cryptosporidium parvum account for the majority of outbreaks (40. 6% and 165; 50. 8%, respectively), Entamoeba histolytica and Cyclospora cayetanensis have been the aetiological agents in nine (2. 8%) and six (1. 8%) outbreaks, respectively, while Toxoplasma gondii and Isospora belli have been responsible for three outbreaks each (0. %) and Blastocystis hominis for two outbreaks (0. 6%). Balantidium coli, the microsporidia, Acanthamoeba and Naegleria fowleri were responsible for one outbreak, each (0. 3%). Moreover, the occurrence of Giardia and Cryptosporidium (oo)cysts in recreational rivers from Malaysia was reported. It was carried out in water samples at two rivers, ‘Sungai Congkak’ and ‘Sungai Batu’, located in Selangor State. The occurrence of both Giardia lamblia and Cryptosporidium parvum (oo)cysts was higher in Sungai Congkak (50% or 15/30 and 10% or 3/30 respectively) than Sungai Batu (16% or 5/30 and 3. % or 1/30 respectively). The mean density of cysts/L was 0. 72 in Sungai Congkak and 0. 023 in Sungai Batu, and that of oocysts/L was 0. 023 in Sungai Congkak and 0. 0033 in Sungai Batu, showing that the occurrence of Giardia was higher and more frequent than Cryptosporidium in both rivers. On the other hand, the Giardia and Cryptosporidium (oo)cysts were more concentrated at the downstream station, followed by midstream and upst ream stations which might be due to human factors where settlements and recreation areas were located around and between midstream and downstream stations.Failure to meet basic human needs for water in China leads to waterborne diseases and preventable deaths, especially among children. The OECD Environmental Indicators in China report issued in July 2007 estimated 30,000 rural children die each year from diarrhea caused by polluted water (OECD, 2007). The World Health Organization reported an incidence of 108. 4 mortalities per 100,000 persons from diarrhea-related illness in China in 2002. In 2003, the National Statistics Office said diarrheal diseases ranked second among the top causes of morbidity in the Philippines with 615,692 cases recorded (World Bank, 2006).Diarrhea is considered a major cause of morbidity, especially in developing countries. In the Philippines, it was the leading cause of morbidity for the years 2001 and 2003, and the second in 2002 (National Statistics Of fice, 2006). Common causes of diarrhea are infections due to protozoa. These causative agents are either foodborne or waterborne. Among enteric protozoa, Giardia lamblia (syn. G. intestinalis or G. duodenalis) and Cryptosporidium spp. are the most commonly reported causes of water-borne diarrhea outbreaks (Natividad et al,2008).The protozoa G. lamblia was first reported in the Philippines in 1977 and since then has been identified as a common intestinal parasite. Studies done in Luzon, in various localities in the Visayas, and in the southern islands of Mindanao indicate wide distribution of Giardia in the Philippines. (Natividad et al, 2008). Stool examinations in the Philippines typically included the identification of the common etiologic agents of diarrheas such as G. lamblia and Cryptosporidium.According to the most recent nationwide survey of Giardia and Cryptosporidium provides basic information on the prevalence of these enteric protozoa in the Philippines. (Buerano et al, 2 008). The worsening water quality is related to incidence of waterborne diseases, and is likely to increase a negative impact of floods on human health. Example of this is an unusual flood that occurred in Marikina City, resulting in high mortality and morbidity rates due to gastroenteritis and other waterborne diseases (The Asian Disaster Preparedness Center, August 2008).The pollution of the surface water in Agusan River could cause a variety of contagious diseases. Those diseases could outbreak through several routes such as: infection by parasites (such as schistosome), which inhabit in the water and drinking of and/or contact with the polluted water river that may cause dysentery and diarrhea (Lomboy, 2011). Instead of developing Davao River as an economic resource, it is currently being used as a central septic tank according to the Davao River Conservation Coordinating Committee.A study conducted by the committee showed that about 11 drainage systems in the city are moving in to the Davao River (Manila Bulletin Publishing Corp. , 2004). Currently, Davao River has become a dumping site for garbage, biological wastes, chemical wastes and other pollutants carried by the residents and even people just within the area (Pascua et. al. , 2011). Thus, areas near or within the urbanized populace such as Bankerohan, Magallanes and Bucana, are considered polluted and may be the source of detrimental effects to those who live nearby. Conceptual FrameworkINDEPENDENT VARIABLEDEPENDENT VARIABLE Fig. 1 Conceptual Paradigm Showing the Relationship of Independent and Dependent Variables of the Study Presented in Fig. 1 above is the conceptual paradigm showing the independent variable, the pathogenic parasites and its levels on the three stations of Davao River namely Bankerohan, Magallanes and Bucana and is the subject of the study. The waterborne diseases and its levels would depend on what pathogenic parasites are found on the three stations of Davao River and is consid ered the dependent variable of the study.Statement of the Problem This study specifically aims to answer the following questions: 1. What is the level of pathogenic parasites in the following stations of Davao River: 1. Bankerohan? 2. Magallanes? 3. Bucana? 2. Is there a significant difference among the levels of pathogenic parasites? 3. What is the level of waterborne diseases in the following stations of Davao River: 1. Bankerohan? 2. Magallanes? 3. Bucana? 4. Is there a significant difference among the levels of waterborne diseases? 5.Is there a significant association between the level of pathogenic parasites and the level of waterborne diseases? Null Hypotheses 1. Hypothesis free 2. There is no significant difference among the levels of pathogenic parasites in the three stations of Davao River. 3. Hypothesis free 4. There is no significant difference among the levels of waterborne diseases in the three stations of Davao River. 5. There is no significant association between the level of pathogenic parasites and the level of waterborne diseases in the three stations of Davao River. Significance of the StudyThe results of this study would provide advantages to various fields namely: Government – They would be able to implement policies and ordinances regarding proper waste management and the dos and don’ts regarding the use of water from the river. The affected residents within the area would be supported and information dissemination within the community would be implemented. Environmental Groups and Agencies – Having been aware on the situation of the 3 stations of Davao River, they would be able to provide solutions to lessen its contamination and prevent increase on water pollution.Community – They would be given health awareness as well as the possible implementation of preventive measures and disease control. Teachers – This study would serve as an educational supplement and could be regarded as a learning material th at could give additional knowledge to those who would desire to conduct a study related to pathogenic organisms found in urbanized rivers. Students – This study could serve as a reference material for further researches. Definition of Terms Davao River. It is one of the largest rivers in the Philippines and the largest in Davao Region.It has a length of 143 kilometers. Salug River located in San Fernando, Bukidnon is its source and the mouth is located at the Davao Gulf. Bacteria. They are single-celled prokaryotic microscopic living organisms that could be pathogenic or likely to cause disease especially waterborne diseases since they could live in water and easily multiply. Parasite. It is an organism that lives in or on another organism (its host) and benefits by getting nutrients directly to its host. It is usually acquired through ingestion of contaminated food or water.Waterborne disease. It is a disease acquired by drinking water contaminated at its source or in the di stribution system, or by direct contact with environmental and recreational waters. It is a disease which results from infection with pathogenic microorganisms or chemical poisoning. Pathogenic microorganisms include viruses, bacteria, protozoans and helminthes. ———————– Waterborne diseases †¢ Bucana †¢ Magallanes †¢ Bankerohan Pathogenic Parasites †¢ Bucana †¢ Magallanes †¢ Bankerohan

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COMPANY PROFILE Company’s Philosophy Our Company’s Main Mission is to provide : * Main quality and best IT Brands * Professional trading standards * Reliable after-sa les service & warranty DISCOUNTPC’S vision is to maintain its status as one premier retail provider – delivering the best I. T. Brands and being known as â€Å"best value for money† retail shop. Contact Information DISCOUNTPC (BEST VALUE MICRO PC) DiscountPC 2nd floor Gilmore I. T. Center Bldg. Gilmore Cor. 1st St. Gilmore, Q. C. PHILS TELS: 722-5018 964-5779 622-3886MOBILE: 0917-8202818 0917-8100560 FAX: 470-9349 EMAIL: [email  protected] com. ph MICROTEQ (MICRO Xchange Tech PC Co. ) MICROTEQ 3RD floor Gilmore I. T. Center Bldg. Gilmore Cor. 1st St. Gilmore, Q. C. Phils TELS: 725-0203 622-0391 MOBILE: 0917-8982310 FAX : 725-0203 EMAIL: [email  protected] com. ph Corporate Information DISCOUNTPC’S vision is to maintain its status as one premier retail provider – deliv ering the best I. T. Brands and being known as â€Å"best value for money† retail shop.The Corporate Head-Office is at the Ground Flr. UNIT101 LEGRAN TOWER NO. 209 EISENHOWER ST. GREENHILLS SAN JUAN, M. M. of which becomes a permanent warehouse because of its 24/7 security convenience and for purposes of an over-all cost cutting rationale. Recently, collections and other office transactions are being made temporarily at another office location at Barcelona TowerII NO. 209 Pasadena Drive San Juan, M. M. The new Corporate Head-Office will be at UNIT 2-I Mezzanine Flr. TRION TOWER I Bonifacio Global City starting October 2012 onwards.Formed in 2002, DiscountPC has been focused from the beginning to maintaining its lead as a retail provider. The company started as MediaHub Accessories Co. in year 1999, Then in 2002, we added three more outlets such as Microteq PC Co. , E-Gear PC Co. , and DiscountPlus PC Co. all at the 2nd flr. Of Virramall before its major renovation in Dec. , 2 004. In 2005, the company transferred to Gilmore area as DISCOUNTPC. However, following the economic crisis in 2008, the company wisely closed two of its branches in Makati (one in Herrera Tower at VA Rufino ST. orner Ayala and another at Blanco Center Leviste St. ). we plan to re-open these two branches before 2012 ends, and a new outlet will be open in GBC on or before 2013. SECRET TO OUT RESILIENCE & SUCCESS Our success is measured by GOOD FUNDINGS, EXCELLENT MANAGEMENT, and FOCUS. It is our ability to converge on customer satisfaction which delivers, our ultimate competency based on trust and loyalty which turns majority of our customers into long term accounts.We will continue to deliver this ATTRIBUTES reliably with expertise and professionalism as we slowly and cautiously evolve to encreasing our outlets in the near future. BRANDS * W. D. â„ ¢ || SEAGATEâ„ ¢ || Seagate Go-Flexâ„ ¢ || Transcendâ„ ¢ || * Kingstonâ„ ¢ || Kingmaxâ„ ¢ || Gailâ„ ¢ || GSkillsâ⠀ž ¢ || Patriotâ„ ¢ || elixirâ„ ¢ * OCZâ„ ¢ agility || OCSâ„ ¢ solid || OCZ â„ ¢ vertex || CORSAIR â„ ¢ || ADATAâ„ ¢ * Thermaltakeâ„ ¢ || Aerocoolâ„ ¢ || Antecâ„ ¢ || InWinâ„ ¢ || Lancoolâ„ ¢ * INTELâ„ ¢ || AMDâ„ ¢ || MICROSOFTâ„ ¢ | NORTONâ„ ¢ || KASPERSKYâ„ ¢ * Gigabyteâ„ ¢ || Asrockâ„ ¢ || EMAXXâ„ ¢ || ASUSâ„ ¢ || Biostarâ„ ¢ || MSIâ„ ¢ * EVGAâ„ ¢ || PalitDaytonaâ„ ¢ || HISâ„ ¢ || Sparklevâ„ ¢ || Afoxâ„ ¢ || Sapphireâ„ ¢ * Samsungâ„ ¢ || AOCâ„ ¢ || HANNS-Gâ„ ¢ || CHIMEIâ„ ¢ || INTEXâ„ ¢ || KTCâ„ ¢ * CANONâ„ ¢ || HPâ„ ¢ || EPSONâ„ ¢ || BROTHERâ„ ¢ || Altecâ„ ¢ || Edifierâ„ ¢ * Dlinkâ„ ¢ || TP-LINKâ„ ¢ || EDIMAXXâ„ ¢ || Logitechâ„ ¢ || A-4TECHâ„ ¢ * MSIâ„ ¢ netbook || ACERâ„ ¢ || NEOâ„ ¢ || ASUSâ„ ¢ MANAGEMENT STAFF Name: MRS. ELIZABETH TANG PARDO – RELUCIO TITLE: MANAGING OWNER AREA: FINANCE / PURCHASING OUTLETS OPERATIONS TEL S: 961-5779 0917-8202818 NAME: MR. ALBER DY RELUCIO TITLE: MANAGING OWNERAREA OF RESPONSIBILITY: AFTER SALES & LOGISTICS TELS: 211-3864 0917-8900306 NAME: MS. ANABELE RIVAS JOB TITLE: MARKETING / PURCHASING TELS: 622-0391 0939-4302891 NAME: MS. JENNIFER PAMING JOB TITLE: ADMIN / PERSONNEL TELS: 622-3886 0917-9100566 NAME: MR. POLICROMIO TACUD JR. JOB TITLE: LOGISTICS TELS: NAME: MS. ROSELYN DELICANA JOB TITLE: INVENTORY CONTROLLER TELS: NAME: MS. VIVIAN ALDEZA JOB TITLE: RMA CONTROLLER TELS: NAME: MR. RONALD SANTOS JOB TITLE: HEAD TECHNICIAN TELS: 725-0203 NAME: ANA MARIE CORRAL JOB TITLE: SALES MANAGER TELS:

Condom Distribution essays

Condom Distribution essays Should Condoms be distributed in High Schools? More than half of American teenagers have had sexual intercourse and face significant health risks. Each year, over one million teenagers become pregnant, one in seven teenagers contract and STD, and one in five hundred students in college campuses are infected with HIV(The National Coalition To Support Sexuality Education). This quote shows why condom distribution should be available in schools. This topic about condom distribution has caused many heated debates around the nation. People who are in favor of distribution will ensure that teenagers practice safe sex and that the rate of STDs and pregnancies will decrease. The opponents say that giving condoms out in high schools would be like encouraging sexual activity among teenagers and put the idea that pre-martial sex is acceptable. So with all of this in mind, is there a need for condom distribution programs? Yes, there is a need for condom distribution programs. Parents and educators seem to be forgetting that there are a great number of teenagers who are sexually active in todays society. The solution to all of this is not a simple one. Deciding whether to put baskets with condoms in the schools nurses office is only one way of getting started with condom distribution programs. But to be more successful its going to require more thought, energy, and a lot of patience, keeping in mind that todays teens are very open minded and independent. Adults are always emphasizing abstinence. Sure abstinence is the best form of birth control and the best way to not contract a STD. But adults also have to realize that all this is not very cool for teens now days. So if they are not going to be able to convince teenagers that abstinence is the best choice, then they should encourage condom distribution in schools, for their own kids safety. The reason why parents find i...

Definition and Examples of Aphesis

Definition and Examples of Aphesis Aphesis is the gradual loss of a short unstressed vowel at the beginning of a word. Adjective: aphetic. Aphesis is commonly regarded as a type of aphaeresis. Compare with apocope and syncope. The opposite of aphesis is prothesis. Generally speaking, aphesis is more common in everyday speech than in formal varieties of spoken and written English. Nonetheless, many aphetic word forms have entered the vocabulary of Standard English. In International English Usage (2005), Todd and Hancock observe that while clipping tends to be rapid and usually applies to the loss of more than one syllable,  aphesis is thought to be a gradual process.   See Examples and Observations below. Also see: AphaeresisClippingEllipsisMetaplasmSound Change EtymologyFrom the Greek, to let go Examples and Observations Cute is an aphetic form of acute; longshore is the truncated form of alongshore. This explains the American usage longshoreman for our [Australian] stevedore. Stevedore is itself an aphetic adaptation of the Spanish estivador, which derives from estivar: to stow a cargo.Likewise, sample is an aphetic form of example; backward is an aphetic form of abackward; and vanguard was once avauntguard, from which avant-garde also derives.Ninny is an aphetic and abbreviated form of an innocent. More recently, we have squire from esquire, specially for especially. In the language of the law, several ambiguous forms survive: vow and avow; void and avoid.(Julian Burnside, Word Watching. Thunders Mouth Press, 2004)The Aphetic  Way as an Intensifier- [As an adverb and intensifier] way is an aphetic form of away; it used to be printed way with an apostrophe, but is rarely so today. It means a great distance or all the way, as in We were way off the mark and We went way to the end of the trolley lin e. Some dictionaries consider this adverbial way colloquial, and indeed it often has a conversational or informal tone, but others consider it appropriate for use at all levels except the most formal or oratorical. It also frequently functions conversationally as an intensifier, as in She was way underprepared for the assignment and the student slang exclamations Way out! Way cool! and the like.(Kenneth G. Wilson,  The Columbia Guide to Standard American English. Columbia University Press, 1993)- I was tired- way tired. I had been on the road- on the run- I dont know- several weeks- a long time.(Andrew Klavan, The Long Way Home. Thomas Nelson, 2010)-  Im really  way too lazy  to try to locate all those ingredients.(Sarah Mlynowski,  Frogs and French Kisses. Delacorte, 2006)-  Ã‚  The widespread, if witless, use of way to mean much or far, very or especially reveals how people favor simplicity over precision, easiness over elegance, popularity over individuality. Its unac ceptable to use this sense of way in your writing, and its unbecoming in your speaking.(Robert Hartwell Fiske,  Robert Hartwell Fiskes Dictionary of Unendurable English. Scribner, 2011) An Aphetic Verbal DoppelgangerDavid Brinkley welcomed Vice President Al Gore on his Sunday morning ABC program with a cordial Thank you for coming. Mr. Goreas so many guests now doanswered with the aphetic thank you with a slight emphasis on the you.Youre welcome used to be the standard response to thank you, writes Daniel Kocan of Orlando, Fla. Now thank you is the stock response to thank you. Since when, and why? Can you explain this recent doppelganger phenomenon?First to doppelganger: this is from the German for the ghostly double of a living person, and is an apt description of the returned thank you. Next to the aphetic, or shortening of words or phrases by the elimination of the unstressed word or syllable: the I is lost in I thank you.(William Safire, On Language: Let Er Rip. The New York Times, November 28, 1993) Pronunciation: AFF-i-sis Also Known As: aphaeresis, apherisis

UNDER THE LION'S PAW by hamlin garland Essay Example | Topics and Well Written Essays - 750 words

UNDER THE LION'S PAW by hamlin garland - Essay Example With almost cinematographic precision the opening paragraph takes the reader in the reality of the farmer’s every day life: IT was the last of autumn and first day of winter coming together. All day long the ploughmen on their prairie farms had moved to and fro in their wide level fields through the falling snow, which melted as it fell, wetting them to the skin all day, notwithstanding the frequent squalls of snow, the dripping, desolate clouds, and the muck of the furrows, black and tenacious as tar (Garland 1). What captures the attention is the literary uniqueness of the characters. Although they are created in order to convey the author’s ideas about the organization of society, the characters in â€Å"Under the Lion’s Paw† also possess captivating dimensionality, which is revealed through their actions, feelings and struggles: The little woman's eyes filled with tears which fell down upon the sleeping baby in her arms. The world was not so desolate a nd cold and hopeless, after all (Garland 3). Garland also conveys broader notions of the organization of society and the distribution of resources through his characters. He expresses his moral views of good and evil. The social contrast between these characters is what constitutes Garland’s universe and the human values on which it is based. One of the leading characters in the story is Timothy Haskins. He is the embodiment of the average, hard-working man, who is ready to sacrifice everything in the name of his ideal, which is his family. Haskins is Garland’s vision of the hard-working, honest man, who has internalized the constraints of the system, which in most of the cases works against him: Many a night he worked till his anxious wife came out at ten o'clock to call him in to rest and lunch [†¦] No slave in the Roman galleys could have toiled so frightfully and lived, for this man thought himself a free man, and that he was working for his wife and babes (Ga rland 10). Timothy Haskins represents the individual aspect of a social struggle, against land speculation and unfair taxation in the 1880s and 1890s. In this sense the story sends the author’s message for social reform as the only road to democratization. The antidote of this vision of social and economic equality is Jim Butler. He is the collective image of those, who have gained economic supremacy through illegal means, and greed has transformed their capacity to create into capacity to dominate. It is difficult to say whether Butler is morally corrupt or just taking advantage of the system. Jim Butler was one of those men called in the West "land poor† [†¦]But a change came over him at the end of the second year, when he sold a lot of land for four times what he paid for it. From that time forward he believed in land speculation as the surest way of getting rich (Garland 5) Finally, Steve Council represents the virtues of society, such as compassion, trustworth iness, sincerity, empathy, honesty, and kindness. These are the pillars of society, as envisioned by the author, which he has embedded in Council’s uncomplicated, noble character. Council moved about uneasily in his seat and stopped his stammering gratitude by saying: "Hold on, now; don't make such a fuss over a little thing. When I see a man down, an' things all on top of 'm, I jest like t' kick 'em